Postdoc function of cytokines in regulating mammalian hematopoietic stem and progenitor cell fates

The Cell Systems Dynamics group studies cell fate choices and their molecular control in complex and dynamic mammalian cell systems.

In the project advertised here, we are interested in the function of cytokines in regulating mammalian hematopoietic stem and progenitor cell fates.

We will use a combination of stem cell purification, culture and manipulation, long-term live cell imaging, single cell tracking and quantification, large volume 3D bone marrrow imaging with single molecule sensitivity, and ai-supported computational analysis.

We are seeking a highly motivated postdoc with expertise in molecular and cellular biology, mammalian blood stem and progenitor cell purification, culture and manipulation, and advanced imaging to join our interdisciplinary team. 

Key Responsibilities:
Quantitative in situ and in vitro imaging studies related to cytokine signaling and cell-cell interactions.
Design and optimization of multiplexed fluorescence imaging, in situ single-molecule detection, and 3D immunofluorescence techniques.
Primary human and mouse stem cell cultures and lentiviral manipulations to study hematopoietic stem cell dynamics.
Primary mammalian tissue handling: isolation, fixation and vibratome sectioning for immunostaining.
AI-assisted image analysis for spatial analysis of tissue samples.
Multidisciplinary team work, with immunologists, data scientists, and bioengineers, to integrate imaging-based techniques into immunotherapy development.
Presentation and publication of research findings.
Supervision of junior researchers to provide technical and scientific training in advanced imaging and molecular techniques.
Contribution to undergraduate student teaching.

Required Qualifications:

Ph.D. in Biotechnology, Immunology, Molecular Biology, Stem Cell Biology or a related field.

Published expertise in:
- mammalian hematopoietic stem cell biology, purification, culture and manipulation.
- molecular biology.
- lenti virus cloning and production.
- live-cell biosensor cloning and use for quantification of cytokine signaling dynamics.
- long-term fluorescence live-cell imaging, single cell tracking and quantification.
- large volume multi-color 3D imaging.
- quantitative computational image analysis.

Track record of high-impact publications.

Experience in teaching (lectures and practical courses).

Experience in Good Laboratory Practice (GLP) and Biosafety Level 2 (BSL2) work.

LTK qualification for mouse work in Switzerland.

• Your career with impact: Become part of ETH Zurich, which not only supports your professional development, but also actively contributes to positive change in society.
• We are actively committed to a sustainable and climate-neutral university.
• You can expect numerous benefits, such as public transport season tickets and car sharing, a wide range of sports offered by the ASVZ, childcare and attractive pension benefits.